Research Scholar, CSIR-IGIB.....
Currency notes are extensively exchanged among individuals in a population and have been studied for their ability to carry and transmit infectious agents. Generally microbial culture-based approaches were used to characterize organisms on currency notes. Culture-based are limited by the conditions and culturing media, which differ widely among different organisms. Previous approaches used 16S ribosomal RNA typing to examine taxonomy of the genetic material isolated. The high-throughput DNA sequencing approach offers advantages in providing additional information on the gene repertoire of organisms on the fomite. This information could provide insights into the catalog of antibiotic resistance genes harboured by these organisms. We show success of our approach by characterizing the microbes, including pathogens on currency notes and additionally provide insights into the antibiotic resistance gene repertoire of the microbes.
Jalali S, Kohli S, Latka C, Bhatia S, Vellarikal SK, Sivasubbu S, Scaria V, Ramachandran S.
Screening currency notes for microbial pathogens and antibiotic resistance genes using a shotgun metagenomic approach.
PLoS One. 2015 Jun 2;10(6):e0128711. doi: 10.1371/journal.pone.0128711. eCollection 2015
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